ʻO ODM Factory Proteinase K CAS No. 39450-01-6 no ka hoʻomaikaʻi ʻana i ka ikaika o ka iwi
ʻO kā mākou kauoha e lawelawe i kā mākou mea hoʻohana a me nā mea kūʻai aku me nā huahana kikohoʻe lawe lima maikaʻi loa a hoʻokūkū no ODM Factory Proteinase K CAS No. 39450-01-6 no ka hoʻomaikaʻi ʻana i ka ikaika o ka iwi, Manaʻo mākou e hauʻoli ʻoe me kā mākou kumukūʻai kūʻai kūpono, nā hoʻonā kiʻekiʻe a me ka lawe wikiwiki ʻana. Ke manaʻolana nei mākou e hiki iā ʻoe ke hāʻawi iā mākou i kahi manawa kūpono e hāʻawi iā ʻoe a lilo i hoa maikaʻi loa iā ʻoe!
ʻO kā mākou kauoha e lawelawe i kā mākou mea hoʻohana a me nā mea kūʻai aku me nā huahana kikohoʻe lawe lima maikaʻi loa a hoʻokūkū noNā Huahana Kemika Kina a me nā Mea Maka, He mau hoʻohālike paʻa lākou a hoʻolaha pono ma ka honua holoʻokoʻa. ʻAʻole loa e nalowale nā hana nui i loko o ka manawa pōkole, he mea pono ia no ʻoe iho o ka maikaʻi maikaʻi loa. Alakaʻi ʻia e ke kumumanaʻo o ka Prudence, Efficiency, Union and Innovation. ke hui. hana i kahi hoʻoikaika maikaʻi loa e hoʻonui i kāna kālepa honua, hoʻonui i kāna ʻoihana. rofit a hoʻonui i kāna unahi hoʻokuʻu aku. Ua hilinaʻi mākou e loaʻa iā mākou kahi hiʻohiʻona mālamalama a e hoʻolaha ʻia ma ka honua holoʻokoʻa i nā makahiki e hiki mai ana.
| Wai helu 1 | |
| Hana kikoʻī protein>800U/mL, ʻo ka nui he 20mg/mL, ʻaʻohe koena Nickase | |
| Pauka helu 2 | |
| helehelena | Pauka lyophilized keʻokeʻo |
| Maʻemaʻe electrophoretic | ≥95% |
| Hana enzyme | ≥30U/mg |
| Koena waikawa nukleika | ʻIke ʻole ʻia |
| Koena Deoxyribonuclease | ʻIke ʻole ʻia |
| Koena Ribonuclease | ʻIke ʻole ʻia |
| Kaʻa kūʻai helu. | G1205-10ML |
| Ka Waihona | Ke mālama ʻia ma –20 °C, mālama ka huahana i ka hana no ka liʻiliʻi he 1 mau makahiki, a laila e lawe ʻia me ka ʻeke hau. |
ʻO Proteinase K kahi serine protease e pili ana i ka ʻohana subtilisin me ka hana enzymatic kūpono a me ka substrate specificity ākea, kahi e makemake ai e hoʻokaʻawale i nā pilina ester a me nā peptides e pili ana i ka C-terminus o nā waikawa amino hydrophobic, nā waikawa amino i loaʻa ka sulfur, a me nā pilina amino acid aromatic, i hoʻohana pinepine ʻia e hoʻohaʻahaʻa i nā protein e hana i nā peptides pōkole. Loaʻa iā Proteinase K ke ʻano o ka triad catalytic maʻamau ʻo Asp39-His69-Ser224 kū hoʻokahi i nā serine proteases a he ʻelua mau wahi hoʻopaʻa Ca2+ a puni ke kikowaena hana e hoʻonui i kona kūpaʻa a mālama i ka hana enzymatic kiʻekiʻe ma lalo o kahi ākea ākea o nā kūlana.
1. Pahu hōʻoia hoʻoilina.
2. Nā pahu hana unuhi RNA a me DNA.
3. Wehe i nā ʻāpana ʻaʻole protein i loko o nā ʻiʻo a hoʻohaʻahaʻa i nā haumia protein (e like me ka hoʻomākaukau ʻana i nā lāʻau pale DNA a me ka heparin).
4. Ka hoʻomākaukau ʻana o ka DNA Chromosomal no ka Pulse Electrophoresis.
5. Ke hoʻomaʻemaʻe ʻana o ke Komohana.
6. Ka hoʻomohala ʻana a me ka hana ʻana o nā reagents albumin glycated enzymatic ma ke kahua o nā diagnostics in vitro.
1. 30mg/ʻōmole 1g/ʻōmole a i ʻole 100g/ʻōmole
2. ʻEke 1kgs/pepa kini
He protease serine paʻa ʻo Proteinase K me ka substrate specificity ākea. Hoʻohaʻahaʻa ia i nā protein he nui i ke kūlana maoli ʻoiai i ke alo o nā detergents. ʻAʻole ia e hoʻāla ʻia e nā ions metala, nā chelating agents (no ka laʻana, EDTA), sulfhydryl reagents, a i ʻole e nā tripsin a i ʻole chymotrypsin inhibitors. He paʻa ia ma luna o kahi laulā pH ākea (4-12.5), me ka hana kūpono ma ka pH 6.5-9.5. Hiki ke hoʻoulu ʻia ka hana ma ka hoʻohui ʻana i nā denaturing agents (SDS a me Urea). Hana ʻia ka denaturation wikiwiki o ka enzyme ma ka mahana ma luna o 70°C. Hana nui ʻia ka autolysis o ka enzyme ma ka pH alkaline. Eia naʻe, ʻaʻole i hoʻopau loa ʻia ʻo Proteinase K e ka autolysis. Ke hoʻomau nei kekahi mau ʻāpana enzyme i kā lākou hana proteolytic piha, ʻoiai ma hope o ka autolysis nui. Hoʻohana pinepine ʻia ʻo Proteinase K i nā noi biology molecular e hoʻoheheʻe i nā protein makemake ʻole ʻia, e like me nā nucleases i loko o nā hoʻomākaukau DNA a i ʻole RNA mai nā microorganisms, nā cell i hoʻoulu ʻia, a me nā mea kanu. Hoʻohana pinepine ʻia ka enzyme ma 50-200 ug/ml i nā hoʻomākaukau waikawa nucleic ma ka pH 7.5-8.0 a me 37 ~ 55oC. ʻOkoʻa nā manawa hoʻomoʻa mai 30 mau minuke a 18 mau hola.
He serine protease paʻa a pane nui hoʻi; hoʻohana ʻia no ka hoʻokaʻawale ʻana i ka protein a me ka nucleic acid. Hoʻohana ʻia ʻo Proteinase K i ka hoʻomaʻemaʻe ʻana o RNA a me DNA mai nā ʻiʻo a i ʻole nā laina cell.
ʻO kā mākou kauoha e lawelawe i kā mākou mea hoʻohana a me nā mea kūʻai aku me nā huahana kikohoʻe lawe lima maikaʻi loa a hoʻokūkū no ODM Factory Proteinase K CAS No. 39450-01-6 no ka hoʻomaikaʻi ʻana i ka ikaika o ka iwi, Manaʻo mākou e hauʻoli ʻoe me kā mākou kumukūʻai kūʻai kūpono, nā hoʻonā kiʻekiʻe a me ka lawe wikiwiki ʻana. Ke manaʻolana nei mākou e hiki iā ʻoe ke hāʻawi iā mākou i kahi manawa kūpono e hāʻawi iā ʻoe a lilo i hoa maikaʻi loa iā ʻoe!
Hale Hana ODMNā Huahana Kemika Kina a me nā Mea Maka, He mau hoʻohālike paʻa lākou a hoʻolaha pono ma ka honua holoʻokoʻa. ʻAʻole loa e nalowale nā hana nui i loko o ka manawa pōkole, he mea pono ia no ʻoe iho o ka maikaʻi maikaʻi loa. Alakaʻi ʻia e ke kumumanaʻo o ka Prudence, Efficiency, Union and Innovation. ke hui. hana i kahi hoʻoikaika maikaʻi loa e hoʻonui i kāna kālepa honua, hoʻonui i kāna ʻoihana. rofit a hoʻonui i kāna unahi hoʻokuʻu aku. Ua hilinaʻi mākou e loaʻa iā mākou kahi hiʻohiʻona mālamalama a e hoʻolaha ʻia ma ka honua holoʻokoʻa i nā makahiki e hiki mai ana.
