hale kūʻai haʻahaʻa kumu kūʻai Alkaline ProteinaseK CAS 39450-01-6
Hoʻopaʻa mākou e hāʻawi i ka helu hoʻokūkū, nā huahana kūʻokoʻa a me nā hoʻonā kiʻekiʻe kiʻekiʻe, e like me ka lawe wikiwiki ʻana no ka hale kūʻai haʻahaʻa Alkaline.ProteinaseK CAS 39450-01-6, Ua makemake nui mākou i mua e launa pū me nā mea kūʻai aku ma nā wahi a pau o ka honua holoʻokoʻa. Manaʻo mākou e māʻona mākou iā ʻoe. Hoʻokipa maikaʻi mākou i nā mea kūʻai aku e hele i kā mākou hui a kūʻai i kā mākou huahana.
Hoʻopaʻa mākou e hāʻawi i ka helu hoʻokūkū, nā huahana kūʻokoʻa a me nā hoʻonā kiʻekiʻe kiʻekiʻe, e like me ka lawe wikiwiki ʻana noKina Alkaline ProteinaseK a me 39450-01-6, Ma muli o ka maika'i maika'i a me nā kumukū'ai kūpono, ua lawe'ia kā mākou mau mea kū'ai i nā'āina a me nā māhele'ē a'e ma mua o 10. Ke kakali nei mākou i ka launa pū ʻana me nā mea kūʻai aku a pau mai ka home a ma waho. Eia kekahi, ʻo ka ʻoluʻolu o ka mea kūʻai aku kā mākou ʻimi mau loa.
| No.1 wai | |
| ʻO ka hana kūikawā o ka protein> 800U / mL,ʻo ka hoʻonuiʻana he 20mg / mL,ʻaʻohe Nickase koena | |
| No.2 pauda | |
| Ka nana aku | ʻO ka pauka lyophilized keʻokeʻo |
| Electrophoric maemae | ≥95% |
| Ka hana enzyme | ≥30U/mg |
| Ke koena nucleic acid | ʻIke ʻole |
| Deoxyribonuclease koena | ʻIke ʻole |
| Ribonuclease koena | ʻIke ʻole |
| Kaʻa No. | G1205-10ML |
| Waihona | Ke mālama ʻia ma –20 °C, mālama ka huahana i ka hana no ka liʻiliʻi o 1 mau makahiki, ka lawe ʻana i ka ʻeke hau. |
ʻO ka protein KHe protease serine no ka ʻohana subtilisin me ka hana enzymatic maikaʻi a me ka kikoʻī o ka substrate ākea, kahi e hoʻoheheʻe ai i nā mea paʻa ester a me nā peptides e pili ana i ka C-terminus o nā waikawa amino hydrophobic, sulfur-containing amino acids, a me nā mea hoʻopaʻa amino acid aromatic. hoʻohana pinepine ʻia e hoʻohaʻahaʻa i nā protein e hana i nā peptides pōkole. Loaʻa i ka Proteinase K ka hiʻohiʻona o ka triad catalytic maʻamau Asp39-His69-Ser224 kū hoʻokahi i nā protease serine a loaʻa iā ia ʻelua mau wahi paʻa Ca2+ a puni ke kikowaena ikaika e hoʻonui i kona kūpaʻa a mālama i ka hana enzymatic kiʻekiʻe ma lalo o kahi ākea ākea.
1. Puke hōʻailona genetic.
2. RNA a me DNA pahu pahu.
3. Wehe i nā ʻāpana protein ʻole i loko o nā ʻiʻo a hoʻohaʻahaʻa i nā haumia protein (e like me ka hoʻomākaukau ʻana i nā kano DNA a me ka heparin).
4. Hoʻomākaukau i ka DNA Chromosomal no ka Pulse Electrophoresis.
5. Ke kāhili komohana.
6. Ka hoʻomohala ʻana a me ka hana ʻana i nā reagents albumin glycated enzymatic ma ke kahua o ka diagnostics in vitro.
1. 30mg /'ōmole 1g /'ōmole a iʻole 100g /'ōmole
2. 1kgs / ʻeke pepa
ʻO ka Proteinase K kahi protease serine kūpaʻa me ka kikoʻī o ka substrate ākea. Hoʻohaʻahaʻa ia i nā protein he nui i ka mokuʻāina maoli a i mua o nā mea holoi. ʻAʻole ia e hoʻāla ʻia e nā ion metala, chelating agents (no ka laʻana, EDTA), sulfhydryl reagents, a i ʻole tripsin a i ʻole chymotrypsin inhibitors. He kūpaʻa ma luna o kahi ākea pH ākea (4-12.5), me ka hana maikaʻi loa ma ka pH 6.5-9.5. Hiki ke hoʻoulu ʻia ka hana ma o ka hoʻohui ʻana i nā mea hoʻokalakupua (SDS a me Urea). Hiki ke denaturation wikiwiki o ka enzyme ma ka mahana ma luna o 70°C. Ke ulu nui nei ka autolysis o ka enzyme ma ka pH alkaline. Eia naʻe, ʻaʻole i hoʻopau ʻia ʻo Proteinase K e ka autolysis. Ke hoʻomau nei kekahi mau ʻāpana enzyme i kā lākou hana proteolytic piha, ʻoiai ma hope o ka autolysis nui. Hoʻohana pinepine ʻia ʻo Proteinase K i nā noi biology molecular e ʻeli i nā protein i makemake ʻole ʻia, e like me nā nucleases i DNA a i ʻole RNA hoʻomākaukau mai nā microorganisms, cell moʻomeheu, a me nā mea kanu. Hoʻohana maʻamau ka enzyme ma 50-200 ug/ml i nā hoʻomākaukau nucleic acid ma pH 7.5-8.0 a me 37~55oC. Hoʻololi nā manawa incubation mai 30 mau minuke a 18 mau hola.
ʻO kahi protease serine kūpaʻa a paʻa loa; hoʻohana ʻia no ka hoʻokaʻawale ʻana i ka protein a me ka nucleic acid. Hoʻohana ʻia ʻo Proteinase K i ka hoʻomaʻemaʻe ʻana i ka RNA a me DNA mai nā ʻiʻo a i ʻole nā laina kelepona.
Kūpaʻa mākou e hāʻawi i ka helu hoʻokūkū, nā huahana kūʻokoʻa a me nā hoʻonā kiʻekiʻe kiʻekiʻe, e like me ka lawe wikiwiki ʻana no ka hale kūʻai haʻahaʻa Alkaline ProteinaseKCAS39450-01-6, Ua makemake nui mākou i mua e launa pū me nā mea kūʻai aku ma nā wahi a pau o ka honua holoʻokoʻa. Manaʻo mākou e māʻona mākou iā ʻoe. Hoʻokipa maikaʻi mākou i nā mea kūʻai aku e hele i kā mākou hui a kūʻai i kā mākou huahana.
kumu kūʻai haʻahaʻaKina Alkaline ProteinaseK a me 39450-01-6, Ma muli o ka maika'i maika'i a me nā kumukū'ai kūpono, ua lawe'ia kā mākou mau mea kū'ai i nā'āina a me nā māhele'ē a'e ma mua o 10. Ke kakali nei mākou i ka launa pū ʻana me nā mea kūʻai aku a pau mai ka home a ma waho. Eia kekahi, ʻo ka ʻoluʻolu o ka mea kūʻai aku kā mākou ʻimi mau loa.
