hale hana haʻahaʻa kumu kūʻai Alkaline ProteinaseK CAS 39450-01-6
Ke kūpaʻa nei mākou e hāʻawi i ka uku hoʻokūkū, nā huahana kūikawā a me nā hoʻonā kiʻekiʻe, a me ka lawe wikiwiki ʻana no ka hale hana haʻahaʻa haʻahaʻa Alkaline.ʻO ka ProteinaseK CAS 39450-01-6, Ua makemake nui mākou e hana pū me nā mea kūʻai aku ma nā wahi āpau o ka honua. Manaʻo mākou e hoʻomāʻona mākou iā ʻoe. Hoʻokipa maikaʻi mākou i nā mea kūʻai aku e kipa i kā mākou hui a kūʻai i kā mākou huahana.
Ke kūpaʻa nei mākou e hāʻawi i ka uku hoʻokūkū, nā huahana kūikawā a me nā hoʻonā kiʻekiʻe, a me ka lawe wikiwiki ʻana noʻO Kina Alkaline ProteinaseK a me 39450-01-6, Ma muli o ke ʻano maikaʻi a me nā kumukūʻai kūpono, ua hoʻokuʻu ʻia aku kā mākou waiwai i nā ʻāina a me nā wahi he 10 a ʻoi. Ke kakali nei mākou e hana pū me nā mea kūʻai aku a pau mai ka home a ma waho. Eia kekahi, ʻo ka ʻoluʻolu o ka mea kūʻai aku kā mākou ʻimi mau loa.
| Wai helu 1 | |
| Hana kikoʻī protein>800U/mL, ʻo ka nui he 20mg/mL, ʻaʻohe koena Nickase | |
| Pauka helu 2 | |
| helehelena | Pauka lyophilized keʻokeʻo |
| Maʻemaʻe electrophoretic | ≥95% |
| Hana enzyme | ≥30U/mg |
| Koena waikawa nukleika | ʻIke ʻole ʻia |
| Koena Deoxyribonuclease | ʻIke ʻole ʻia |
| Koena Ribonuclease | ʻIke ʻole ʻia |
| Kaʻa kūʻai helu. | G1205-10ML |
| Ka Waihona | Ke mālama ʻia ma –20 °C, mālama ka huahana i ka hana no ka liʻiliʻi he 1 mau makahiki, a laila e lawe ʻia me ka ʻeke hau. |
ʻO ka proteinase KʻO ia kahi serine protease e pili ana i ka ʻohana subtilisin me ka hana enzymatic kūpono a me ka substrate specificity ākea, kahi e hoʻokaʻawale ai i nā pilina ester a me nā peptides e pili ana i ka C-terminus o nā waikawa amino hydrophobic, nā waikawa amino i loaʻa ka sulfur, a me nā pilina amino acid aromatic, i hoʻohana pinepine ʻia e hoʻohaʻahaʻa i nā protein e hana i nā peptides pōkole. Loaʻa iā Proteinase K ke ʻano o ka triad catalytic maʻamau ʻo Asp39-His69-Ser224 kū hoʻokahi i nā serine proteases a he ʻelua mau wahi hoʻopaʻa Ca2+ a puni ke kikowaena hana e hoʻonui i kona kūpaʻa a mālama i ka hana enzymatic kiʻekiʻe ma lalo o kahi ākea ākea o nā kūlana.
1. Pahu hōʻoia hoʻoilina.
2. Nā pahu hana unuhi RNA a me DNA.
3. Wehe i nā ʻāpana ʻaʻole protein i loko o nā ʻiʻo a hoʻohaʻahaʻa i nā haumia protein (e like me ka hoʻomākaukau ʻana i nā lāʻau pale DNA a me ka heparin).
4. Ka hoʻomākaukau ʻana o ka DNA Chromosomal no ka Pulse Electrophoresis.
5. Ke hoʻomaʻemaʻe ʻana o ke Komohana.
6. Ka hoʻomohala ʻana a me ka hana ʻana o nā reagents albumin glycated enzymatic ma ke kahua o nā diagnostics in vitro.
1. 30mg/ʻōmole 1g/ʻōmole a i ʻole 100g/ʻōmole
2. ʻEke 1kgs/pepa kini
He protease serine paʻa ʻo Proteinase K me ka substrate specificity ākea. Hoʻohaʻahaʻa ia i nā protein he nui i ke kūlana maoli ʻoiai i ke alo o nā detergents. ʻAʻole ia e hoʻāla ʻia e nā ions metala, nā chelating agents (no ka laʻana, EDTA), sulfhydryl reagents, a i ʻole e nā tripsin a i ʻole chymotrypsin inhibitors. He paʻa ia ma luna o kahi laulā pH ākea (4-12.5), me ka hana kūpono ma ka pH 6.5-9.5. Hiki ke hoʻoulu ʻia ka hana ma ka hoʻohui ʻana i nā denaturing agents (SDS a me Urea). Hana ʻia ka denaturation wikiwiki o ka enzyme ma ka mahana ma luna o 70°C. Hana nui ʻia ka autolysis o ka enzyme ma ka pH alkaline. Eia naʻe, ʻaʻole i hoʻopau loa ʻia ʻo Proteinase K e ka autolysis. Ke hoʻomau nei kekahi mau ʻāpana enzyme i kā lākou hana proteolytic piha, ʻoiai ma hope o ka autolysis nui. Hoʻohana pinepine ʻia ʻo Proteinase K i nā noi biology molecular e hoʻoheheʻe i nā protein makemake ʻole ʻia, e like me nā nucleases i loko o nā hoʻomākaukau DNA a i ʻole RNA mai nā microorganisms, nā cell i hoʻoulu ʻia, a me nā mea kanu. Hoʻohana pinepine ʻia ka enzyme ma 50-200 ug/ml i nā hoʻomākaukau waikawa nucleic ma ka pH 7.5-8.0 a me 37 ~ 55oC. ʻOkoʻa nā manawa hoʻomoʻa mai 30 mau minuke a 18 mau hola.
He serine protease paʻa a pane nui hoʻi; hoʻohana ʻia no ka hoʻokaʻawale ʻana i ka protein a me ka nucleic acid. Hoʻohana ʻia ʻo Proteinase K i ka hoʻomaʻemaʻe ʻana o RNA a me DNA mai nā ʻiʻo a i ʻole nā laina cell.
Ke kūpaʻa nei mākou e hāʻawi i ka uku hoʻokūkū, nā huahana kūikawā a me nā hoʻonā kiʻekiʻe kiʻekiʻe, a me ka lawe wikiwiki ʻana no ke kumu kūʻai haʻahaʻa o ka hale hana Alkaline ProteinaseK.CAS39450-01-6, Ua makemake nui mākou e hana pū me nā mea kūʻai aku ma nā wahi āpau o ka honua. Manaʻo mākou e hoʻomāʻona mākou iā ʻoe. Hoʻokipa maikaʻi mākou i nā mea kūʻai aku e kipa i kā mākou hui a kūʻai i kā mākou huahana.
hale hana haʻahaʻa kumukūʻaiʻO Kina Alkaline ProteinaseK a me 39450-01-6, Ma muli o ke ʻano maikaʻi a me nā kumukūʻai kūpono, ua hoʻokuʻu ʻia aku kā mākou waiwai i nā ʻāina a me nā wahi he 10 a ʻoi. Ke kakali nei mākou e hana pū me nā mea kūʻai aku a pau mai ka home a ma waho. Eia kekahi, ʻo ka ʻoluʻolu o ka mea kūʻai aku kā mākou ʻimi mau loa.
