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ʻO ka hale kūʻai maikaʻi loa ke kūʻai aku nei i ka Globally Popular Proteinase K CAS 39450-01-6 me kahi kiʻekiʻe o ka Exposure Rate a me ka lā hou o ka hana ʻana.


  • Helu CAS:39450-01-6
  • Nā inoa ʻē aʻe:ʻO Proteinase K, ProteinaseK, PaukaA me ka Hoʻonā Loaʻa; ProteinaseK, mai TritirachiulbumLimber; ProteinaseKfromTritirachiulbumca.8DMC-U/mg; PROTEINASEK, LYOPHILIZEDPOWDER, BCchemicalbookIOTECHGRADE; ProteinaseKfortritirachiulbumserine.
  • MF:C29H27N2O12P
  • Helu EINECS:254-457-8
  • Huahana Huahana

    Hoʻoiho

    Huahana Huahana

    Hoʻohanohano kēlā me kēia lālā o kā mākou hui kālā hana nui i nā koi o nā mea kūʻai aku a me ka kamaʻilio hui ʻana no Factory kūʻai maikaʻi loa ma ka honuaProteinase K CAS 39450-01-6me ka High Exposure Rate and The Latest Date of Production, I kēia manawa, ke kakali nei mākou i ka hui nui ʻana me nā mea kūʻai aku ma waho e pili ana i nā pono like. E hōʻoia ʻoe ʻaʻohe kumu kūʻai e launa pū me mākou no ka ʻike hou aku.
    Hoʻohanohano kēlā me kēia lālā o kā mākou hui kālā hana nui i nā koi o nā mea kūʻai aku a me ka kamaʻilio hui noKina 39450-01-6, Hoʻokipa maikaʻi mākou i kāu kākoʻo a lawelawe mākou i kā mākou mea kūʻai aku ma ka home a ma waho me nā waiwai o ka maikaʻi a me ka lawelawe maikaʻi loa i hoʻonohonoho ʻia i ke ʻano o ka hoʻomohala hou e like me nā manawa a pau. Manaʻo mākou e pōmaikaʻi ʻoe mai kā mākou ʻoihana ʻoihana koke.

    No.1 wai

    ʻO ka hana kūikawā o ka protein> 800U / mL,ʻo ka hoʻonuiʻana he 20mg / mL,ʻaʻohe Nickase koena

    No.2 pauda

    Ka nana aku ʻO ka pauka lyophilized keʻokeʻo
    Electrophoric maemae ≥95%
    Ka hana enzyme ≥30U/mg
    Ke koena nucleic acid ʻIke ʻole
    Deoxyribonuclease koena ʻIke ʻole
    Ribonuclease koena ʻIke ʻole
    Kaʻa No. G1205-10ML
    Waihona Ke mālama ʻia ma –20 °C, mālama ka huahana i ka hana no ka liʻiliʻi o 1 mau makahiki, ka lawe ʻana i ka ʻeke hau.

    ʻO ka protein KHe protease serine no ka ʻohana subtilisin me ka hana enzymatic maikaʻi a me ka kikoʻī o ka substrate ākea, kahi e hoʻoheheʻe ai i nā mea paʻa ester a me nā peptides e pili ana i ka C-terminus o nā waikawa amino hydrophobic, sulfur-containing amino acids, a me nā mea hoʻopaʻa amino acid aromatic. hoʻohana pinepine ʻia e hoʻohaʻahaʻa i nā protein e hana i nā peptides pōkole. Loaʻa i ka Proteinase K ka hiʻohiʻona o ka triad catalytic maʻamau Asp39-His69-Ser224 kū hoʻokahi i nā protease serine a loaʻa iā ia ʻelua mau wahi paʻa Ca2+ a puni ke kikowaena ikaika e hoʻonui i kona kūpaʻa a mālama i ka hana enzymatic kiʻekiʻe ma lalo o kahi ākea ākea.

    1. Puke hōʻailona genetic.
    2. RNA a me DNA pahu pahu.
    3. Wehe i nā ʻāpana protein ʻole i loko o nā ʻiʻo a hoʻohaʻahaʻa i nā haumia protein (e like me ka hoʻomākaukau ʻana i nā kano DNA a me ka heparin).
    4. Hoʻomākaukau i ka DNA Chromosomal no ka Pulse Electrophoresis.
    5. Ke kāhili komohana.
    6. Ka hoʻomohala ʻana a me ka hana ʻana i nā reagents albumin glycated enzymatic ma ke kahua o ka diagnostics in vitro.

    1. 30mg /'ōmole 1g /'ōmole a iʻole 100g /'ōmole
    2. 1kgs / ʻeke pepa

    ʻO ka Proteinase K kahi protease serine kūpaʻa me ka kikoʻī o ka substrate ākea. Hoʻohaʻahaʻa ia i nā protein he nui i ka mokuʻāina maoli a i mua o nā mea holoi. ʻAʻole ia e hoʻāla ʻia e nā ion metala, chelating agents (no ka laʻana, EDTA), sulfhydryl reagents, a i ʻole tripsin a i ʻole chymotrypsin inhibitors. He kūpaʻa ma luna o kahi ākea pH ākea (4-12.5), me ka hana maikaʻi loa ma ka pH 6.5-9.5. Hiki ke hoʻoulu ʻia ka hana ma o ka hoʻohui ʻana i nā mea hoʻokalakupua (SDS a me Urea). Hiki ke denaturation wikiwiki o ka enzyme ma ka mahana ma luna o 70°C. Ke ulu nui nei ka autolysis o ka enzyme ma ka pH alkaline. Eia naʻe, ʻaʻole i hoʻopau ʻia ʻo Proteinase K e ka autolysis. Ke hoʻomau nei kekahi mau ʻāpana enzyme i kā lākou hana proteolytic piha, ʻoiai ma hope o ka autolysis nui. Hoʻohana pinepine ʻia ʻo Proteinase K i nā noi biology molecular e ʻeli i nā protein i makemake ʻole ʻia, e like me nā nucleases i DNA a i ʻole RNA hoʻomākaukau mai nā microorganisms, cell moʻomeheu, a me nā mea kanu. Hoʻohana maʻamau ka enzyme ma 50-200 ug/ml i nā hoʻomākaukau nucleic acid ma pH 7.5-8.0 a me 37~55oC. Hoʻololi nā manawa incubation mai 30 mau minuke a 18 mau hola.

    ʻO kahi protease serine kūpaʻa a paʻa loa; hoʻohana ʻia no ka hoʻokaʻawale ʻana i ka protein a me ka nucleic acid. Hoʻohana ʻia ʻo Proteinase K i ka hoʻomaʻemaʻe ʻana i ka RNA a me DNA mai nā ʻiʻo a i ʻole nā ​​laina kelepona.

    Hoʻohanohano kēlā me kēia lālā o kā mākou hui kālā hana nui i nā koi o nā mea kūʻai aku a me ka kamaʻilio hui ʻana no Factory kūʻai maikaʻi loa i ka Globally Popular Proteinase K CAS39450-01-6me ka High Exposure Rate and The Latest Date of Production, I kēia manawa, ke kakali nei mākou i ka launa pū ʻana me nā mea kūʻai aku ma waho e pili ana i nā pono like. E hōʻoia ʻoe ʻaʻohe kumu kūʻai e launa pū me mākou no ka ʻike hou aku.
    ʻO ka hale kūʻai maikaʻi loaKina 39450-01-6, Hoʻokipa maikaʻi mākou i kāu kākoʻo a lawelawe mākou i kā mākou mea kūʻai aku ma ka home a me nā ʻāina ʻē me nā waiwai ʻoi aku ka maikaʻi a me ka lawelawe maikaʻi loa i hoʻonohonoho ʻia i ke ʻano o ka hoʻomohala hou e like me nā manawa a pau. Manaʻo mākou e pōmaikaʻi ʻoe mai kā mākou ʻoihana ʻoihana koke.


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